rabbit polyclonal anti glua1 (Millipore)
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Millipore
rabbit polyclonal anti glua1
Rabbit Polyclonal Anti Glua1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti-glua1/glua1+antibody/pmc12013928-2-0-5
Average 90 stars, based on 1 article reviews
Rabbit Polyclonal Anti Glua1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti-glua1/glua1+antibody/pmc12013928-2-0-5
Average 90 stars, based on 1 article reviews
rabbit polyclonal anti glua1 - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Astrocyte glypican 5 regulates synapse maturation and stabilization"
Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization
Journal: Cell reports
doi: 10.1016/j.celrep.2025.115374
Figure Legend Snippet: (A–D) GLUA2 protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .
Techniques Used: Immunostaining
Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Recombinant, Modification, Saline, RNAscope, Multiplex Assay, Electron Microscopy, Negative Control, Software, Cell Culture, Fluorescence, Microscopy, Hybridization
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Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , Protease Inhibitor:Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons Article Snippet: Secondary antibodies in Triton/NGS/PBS were applied for 1 h. The following primary antibodies were used: rabbit polyclonal anti-Prr7 (200 ng/ml, PA5-61266; Invitrogen), chicken monoclonal anti-Map2 (1:5,000 dilution, PA1-16751; Thermo Fisher Scientific), rabbit polyclonal anti-SPAR (1:1,500 dilution, kind gift of DT Pak), Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton. Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , Plasmid Preparation:Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons Article Snippet: Secondary antibodies in Triton/NGS/PBS were applied for 1 h. The following primary antibodies were used: rabbit polyclonal anti-Prr7 (200 ng/ml, PA5-61266; Invitrogen), chicken monoclonal anti-Map2 (1:5,000 dilution, PA1-16751; Thermo Fisher Scientific), rabbit polyclonal anti-SPAR (1:1,500 dilution, kind gift of DT Pak), Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton. Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , Enzyme-linked Immunosorbent Assay:Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons Article Snippet: Secondary antibodies in Triton/NGS/PBS were applied for 1 h. The following primary antibodies were used: rabbit polyclonal anti-Prr7 (200 ng/ml, PA5-61266; Invitrogen), chicken monoclonal anti-Map2 (1:5,000 dilution, PA1-16751; Thermo Fisher Scientific), rabbit polyclonal anti-SPAR (1:1,500 dilution, kind gift of DT Pak), Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton. Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , Bicinchoninic Acid Protein Assay:Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons Article Snippet: Secondary antibodies in Triton/NGS/PBS were applied for 1 h. The following primary antibodies were used: rabbit polyclonal anti-Prr7 (200 ng/ml, PA5-61266; Invitrogen), chicken monoclonal anti-Map2 (1:5,000 dilution, PA1-16751; Thermo Fisher Scientific), rabbit polyclonal anti-SPAR (1:1,500 dilution, kind gift of DT Pak), Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton. Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , Software:Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons Article Snippet: Secondary antibodies in Triton/NGS/PBS were applied for 1 h. The following primary antibodies were used: rabbit polyclonal anti-Prr7 (200 ng/ml, PA5-61266; Invitrogen), chicken monoclonal anti-Map2 (1:5,000 dilution, PA1-16751; Thermo Fisher Scientific), rabbit polyclonal anti-SPAR (1:1,500 dilution, kind gift of DT Pak), Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton. Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , Imaging:Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons Article Snippet: Secondary antibodies in Triton/NGS/PBS were applied for 1 h. The following primary antibodies were used: rabbit polyclonal anti-Prr7 (200 ng/ml, PA5-61266; Invitrogen), chicken monoclonal anti-Map2 (1:5,000 dilution, PA1-16751; Thermo Fisher Scientific), rabbit polyclonal anti-SPAR (1:1,500 dilution, kind gift of DT Pak), Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton. Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , Inverted Microscopy:Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons Article Snippet: Secondary antibodies in Triton/NGS/PBS were applied for 1 h. The following primary antibodies were used: rabbit polyclonal anti-Prr7 (200 ng/ml, PA5-61266; Invitrogen), chicken monoclonal anti-Map2 (1:5,000 dilution, PA1-16751; Thermo Fisher Scientific), rabbit polyclonal anti-SPAR (1:1,500 dilution, kind gift of DT Pak), Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton. Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , Microscopy:Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons Article Snippet: Secondary antibodies in Triton/NGS/PBS were applied for 1 h. The following primary antibodies were used: rabbit polyclonal anti-Prr7 (200 ng/ml, PA5-61266; Invitrogen), chicken monoclonal anti-Map2 (1:5,000 dilution, PA1-16751; Thermo Fisher Scientific), rabbit polyclonal anti-SPAR (1:1,500 dilution, kind gift of DT Pak), Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton. Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , Virus:Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons Article Snippet: Secondary antibodies in Triton/NGS/PBS were applied for 1 h. The following primary antibodies were used: rabbit polyclonal anti-Prr7 (200 ng/ml, PA5-61266; Invitrogen), chicken monoclonal anti-Map2 (1:5,000 dilution, PA1-16751; Thermo Fisher Scientific), rabbit polyclonal anti-SPAR (1:1,500 dilution, kind gift of DT Pak), Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton. Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , Mass Spectrometry:Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons Article Snippet: Secondary antibodies in Triton/NGS/PBS were applied for 1 h. The following primary antibodies were used: rabbit polyclonal anti-Prr7 (200 ng/ml, PA5-61266; Invitrogen), chicken monoclonal anti-Map2 (1:5,000 dilution, PA1-16751; Thermo Fisher Scientific), rabbit polyclonal anti-SPAR (1:1,500 dilution, kind gift of DT Pak), Article Title: Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton. Article Snippet: REAGENT or Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: Article Title: Absence of chordin-like 1 aids motor recovery in a mouse model of stroke Article Snippet: Sections were then incubated with the corresponding primary antibodies: Article Title: Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-AnkB NeuroMab Cat#75–144; RRID: AB_10673094 Rabbit polyclonal anti-Kalirin MilliPORE SiGMA Cat#07–122; RRID: AB 310380 Rabbit polyclonal anti-RPS4X Proteintech Cat# 14799-1-AP; RRID: AB 2238567 Mouse monoclonal anti-Sodium Potassium ATPase Alpha 1 NOVUS BIOLOGICALS Cat# NB300-146; RRID: AB 2060981 Rabbit monoclonal anti-NF-L Abcam Cat# ab223343; RRID: AB 2891198 Rabbit monoclonal anti-GAPDH Cell Signaling Cat# 5174; RRID: AB 10622025 Rabbit polyclonal anti-Shank3 Synaptic Systems Cat#162 302; RRID: AB_2619862 Rabbit polyclonal anti-GluA1 MilliPORE SiGMA Cat#AB1504; RRID: AB_2113602 Rabbit polyclonal anti-GluA2 MilliPORE SiGMA Cat#AB10529; RRID: AB_10807819 Mouse monoclonal anti-AnkG NeuroMab Cat#75–146; RRID: AB_10673030 Goat anti-mouse 647 Thermo Fisher Scientific Cat#A-21134; RRID: AB_2535773 Bacterial and virus strains E. coli HST08 TaKaRa Cat#636766 Chemicals, peptides, and recombinant proteins Perampanel Toronto Research Chemicals Cat#P285520 Article Title: Disuse plasticity limits spinal cord injury recovery Article Snippet: Rabbit Polyclonal Anti-GluA1 , |
